Finally, a fed-batch fermentation in a 150-liter fermentor was established to prepare GAMG through GL hydrolysis by T. Homology modeling, substrate docking, and sequence alignment were employed to identify the key residues for substrate recognition. TpGUS79A showed a much higher catalytic efficiency on GL ( k cat/ K m of 11.14 mM −1 s −1) than on the artificial substrate pNP β-glucopyranosiduronic acid ( k cat/ K m of 0.01 mM −1 s −1), which is different from the case for most GUSs. Interestingly, TpGUS79A recognized and hydrolyzed the distal glucuronic bond of GL but could not cleave the glucuronic bond in GAMG. TpGUS79A had strict specificity toward the glycan moiety but poor specificity toward the aglycone moiety. TpGUS79A belonged to glycoside hydrolase family 79 (GH79) but shared low amino acid sequence identity (<35%) with the available GH79 GUS enzymes. The gene for TpGUS79A was then cloned and verified by heterologous expression in Pichia pastoris. First, TpGUS79A was purified and identified through matrix-assisted laser desorption ionization–tandem time of flight mass spectrometry (MALDI-TOF-TOF MS) and deglycosylation, indicating that TpGUS79A is a highly N-glycosylated monomeric protein with a molecular mass of around 85 kDa, including around 25 kDa of glycan moiety. In this study, we isolated a β-glucuronidase (TpGUS79A) from the filamentous fungus Talaromyces pinophilus Li-93 that can specifically and precisely convert GL to GAMG without the formation of the by-product glycyrrhetinic acid (GA) from the further hydrolysis of GAMG. Wondering what has changed in the latest releases? Have a look at the change log.Glycyrrhetinic acid 3- O-mono-β- d-glucuronide (GAMG), which possesses a higher sweetness and stronger pharmacological activity than those of glycyrrhizin (GL), can be obtained by removal of the distal glucuronic acid (GlcA) from GL. SVG.js is licensed under the terms of the MIT License. fully documented (you're looking at it :).
#Svgus 1 full#
full support for opacity masks and clipping paths.unified api between shape types with move, size, center.painless extension thanks to the modular structure.animations on size, position, transformations, color.
#Svgus 1 code#
That's just two lines of code instead of ten! And a whole lot less repetition. Doing the same as the vanilla js example above: // SVG.js SVG.js provides a syntax that is concise and easy to read. For example, just creating a simple pink square requires quite a lot of code:
Tested on an Intel Core i7-4702MQ 2.2GHz.Ĭreating and manipulating SVG using JavaScript alone is pretty verbose. gradient: generate 10000 rects with gradient fill.
0 kommentar(er)
